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- microfossil analysis –1 mg of foodcrust,
- Proteomic analysis – 10 mg of foodcrust,
- bulk stable isotopeanalysis(with EA-IRMS) – ca 5 mg offoodcrust;
- lipid residue analysis, ca 20 mg foodcrust; at least 0.5 g of homogenised ceramic powder for acid extraction, and around 1 g for solvent extraction.
Proteomics Data Workflow
Data Acquisition and Export
Data Processing with LC-MS
Analysis Scripts and Computational Tools (MaxQuant, MetaMorpheus
Lipid Data Workflow
In lipid analysis we usually have three steps of workflow generating a series of raw data -> interpreted data -> integrated data.
- Data Acquisition and Export (GC-FID, GC-MS, GC-SIM, GC-C-IRMS)
GC-FID and GC-MS (scan and SIM) data is generated by masshunter software.
For example, there is a sequence of measurements.
Each measurement will generate a folder containing a series of raw data (method acquisition file and data files).
GC-C-IRMS data is generated by Qtegra.
Each sequence produces a single container file (.imexp) that contains all the measurement data. It is not possible to extract individual measurements from this file. The .imexp format can only be opened with Qtegra® software.
After initial checks and corrections in Qtegra®, the relevant data is exported to .xlsx format for calibration and for general accessibility.
It's also possible to export all chromatogram data points, allowing graphs to be reconstructed in Excel (.xlsx) or ohter programs as well.
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Data reliability/limitation of method
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Data Workflow
Lipid Data Workflow
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Microfossil Data Workflow
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